Purification of Prenylated Methylated Protein Methyl Esterase from Porcine Liver Supernatant

Polyisoprenylated methylated protein methyl esterase as a putative drug target for androgen-insensitive prostate cancer

Prostate cancer (CaP) is the most frequently diagnosed cancer in US men, with an estimated 236,590 new cases and 29,720 deaths in 2013. There exists the need to identify biomarkers/therapeutic targets for the early/companion diagnosis and development of novel therapies against the recalcitrant disease. Mutation and overexpression-induced abnormal activities of polyisoprenylated proteins have be...

Purification and characterization of acylation stimulating protein from porcine serum.

A method for purifying acylation stimulating protein (ASP) from porcine serum is described. The mRNA encoding ASP was cloned by reverse transcriptase-polymerase chain reaction which predicted a 76 residue peptide. Based on this sequence, we generated antisera to a C-terminal peptide (ASP(1-20)) which aided ASP purification. Identity of the purified protein was verified by N-terminal sequencing....

Polyisoprenylated Methylated Protein Methyl Esterase Is Both Sensitive to Curcumin and Overexpressed in Colorectal Cancer: Implications for Chemoprevention and Treatment

Inhibition of PMPMEase, a key enzyme in the polyisoprenylation pathway, induces cancer cell death. In this study, purified PMPMEase was inhibited by the chemopreventive agent, curcumin, with a K(i) of 0.3 μM (IC50 = 12.4 μM). Preincubation of PMPMEase with 1 mM curcumin followed by gel-filtration chromatography resulted in recovery of the enzyme activity, indicative of reversible inhibition. Ki...

Protein purification from whole tissues . Fatemeh Mehrpooyan*

Purification of a ribonuclease inhibitor from Novikoff hepatoma supernatant fraction.

A comparison between the levels of ribonuclease inhibitor in normal rat liver and Novikoff hepatoma 105,000 X g supernatant fractions is reported. Novikoff hepatoma con tained 61% less inhibitor per g of tissue or 39% less per mg protein than did normal rat liver. A rapid and simple method that had been developed for the purification of rat liver ribonuclease inhibitor was applied to Novikoff h...